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Updated: May 13, 2025

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Protocol for measuring membrane elasticity of mouse cardiomyocytes using atomic force microscopy.

Andrés David Morales Maldonado1, Daphne Agostina Diloretto2, Valeriy Timofeyev2

  • 1Department of Chemistry, University of California, Davis, Davis, CA 95616, USA.

STAR Protocols
|April 13, 2025
PubMed
Summary

Atomic force microscopy (AFM) provides a method to measure single-cell mechanics. This study details using AFM to quantify the membrane mechanical properties of mouse ventricular cardiomyocytes.

Keywords:
Atomic Force Microscopy (AFM)BiophysicsCell BiologyCell isolationHealth SciencesSingle Cell

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Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Cellular mechanics are crucial for understanding cell function.
  • Atomic force microscopy (AFM) is a key technique for single-cell mechanical analysis.
  • Cardiomyocytes possess unique mechanical properties essential for heart function.

Purpose of the Study:

  • To present a detailed protocol for measuring the mechanical properties of cardiomyocyte membranes using AFM.
  • To quantify the membrane Young's moduli of mouse ventricular cardiomyocytes.
  • To enhance understanding of cardiomyocyte biomechanics.

Main Methods:

  • Isolation of mouse ventricular cardiomyocytes.
  • Preparation, modification, and calibration of AFM cantilevers.
  • Measurement of force-deformation profiles to determine membrane Young's moduli.

Main Results:

  • A standardized protocol for AFM-based measurement of cardiomyocyte membrane mechanics was established.
  • Quantification of membrane Young's moduli for mouse ventricular cardiomyocytes was achieved.
  • The study provides insights into the mechanical characteristics of these cells.

Conclusions:

  • AFM is a suitable technique for assessing cardiomyocyte membrane mechanics.
  • The developed protocol facilitates reproducible mechanical property measurements.
  • This research contributes to the understanding of cardiac cell biomechanics.