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Related Experiment Video

Updated: May 13, 2025

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry CCMS
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Simultaneous Quantitative Detection of Cysteine and Homocysteine Labeled by 1-Pyrenecarboxaldehyde Using MALDI-TOF

Liming Guo1,2, Hao Wang3, Liang Song2

  • 1State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.

Journal of the American Society for Mass Spectrometry
|April 15, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new method using 1-pyrenecarboxaldehyde for quantifying cysteine (Cys) and homocysteine (Hcy) via MALDI-TOF MS. The approach achieves high sensitivity and accuracy in biological samples without internal standards.

Keywords:
CysteineHomocysteineMatrix-Assisted Laser Desorption/Ionization Mass SpectrometrySimultaneous Quantification

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Cysteine (Cys) and homocysteine (Hcy) are vital biothiols involved in numerous physiological processes.
  • Accurate quantification of Cys and Hcy is crucial for understanding their biological roles.

Purpose of the Study:

  • To develop a sensitive and accurate method for the simultaneous quantification of Cys and Hcy.
  • To utilize 1-pyrenecarboxaldehyde (1-py) derivatization for enhanced detection via MALDI-TOF MS.

Main Methods:

  • Derivatization of Cys and Hcy with 1-pyrenecarboxaldehyde (1-py).
  • Quantitative analysis using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS).
  • Method validation including detection limits, linear ranges, and application to cell lysate.

Main Results:

  • Achieved a low detection limit of 250 amol/L for Cys and Hcy.
  • Established linear quantitative ranges from 5 to 2500 μM without internal standards.
  • Successfully quantified Cys and Hcy in HepG2 cell lysate.

Conclusions:

  • The 1-py derivatization method coupled with MALDI-TOF MS provides a rapid, sensitive, and accurate approach for biothiol quantification.
  • This method offers a valuable tool for biological and clinical research involving Cys and Hcy.