Related Experiment Video
Updated: May 5, 2026

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
Changes of Barley Bound Phenolics and Their Characteristics During Simulated Gastrointestinal Digestion and Colonic
Yansheng Zhao1, Fei Leng1, Songtao Fan1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Abstract:
Phenolic compounds in cereals, known for their biological activities, are primarily found in a bound state within the bran. Their changes during digestion are linked to physiological activities. In this study, the dynamic changes and fermentation characteristics of barley bound phenolics (BBPs) were investigated through an in vitro rat gastrointestinal digestion and colonic fermentation. UPLC-HRMS revealed that the release rate of BBPs during colonic fermentation was significantly higher than that during gastric digestion (0.13%) and intestinal digestion (0.43%), reaching 5.02%. After 48 h of colonic fermentation, gallic acid and ferulic acid accounted for 35.05% and 27.84% of the total released phenolic acids, respectively. Confocal microscopy confirmed that BBPs were predominantly released in the colon. Additionally, BBPs significantly increased the content of acetate during colonic fermentation compared to the control samples, correlating with a decrease in pH value. 16S rRNA sequencing further revealed the modulatory effects of BBPs on colonic microbiota structure: BBPs significantly enhanced the Chao1 and Shannon indices of the microbiota. Notably, BBPs inhibited the growth of potentially harmful bacteria such as Proteobacteria and Enterobacteriaceae while promoting the proliferation of beneficial bacteria such as Akkermansia and Bifidobacteriaceae, thereby modulating the structure of the gut microbial community. These findings suggested that BBPs may promote gut health through prebiotic activity in the colon.
More Related Videos
Related Concept Videos
Carbohydrate Digestion
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Microbes in Beverage Production
Microbes in the Production of Fermented Foods

