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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Engineering coupled consortia-based biosensors for diagnostic.

Rongying Huang¹, Valeriia Kravchik², Rawan Zaatry³

¹Department of Biotechnology Technion-Israel Institute of Technology, Technion City, Haifa, Israel.

Nature Communications

|April 22, 2025

Summary

This summary is machine-generated.

Engineered bacterial consortia can detect Heme and Lactate using a shared signal. An incoherent feedforward loop (IFFL) system provides robust and stable biosensing for diagnostics.

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BioMEMS: Forging New Collaborations Between Biologists and Engineers

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Published on: November 1, 2007

Area of Science:

Synthetic biology
Microbial consortia engineering
Biosensor development

Background:

Synthetic multicellular systems offer advanced capabilities like multi-signal detection and computation via cell-to-cell communication.
Engineering these systems requires precise control over cell concentrations and coordinated activity.
Bacterial consortia present a promising platform for complex biological tasks.

Purpose of the Study:

To develop a bacterial consortia-based biosensor for simultaneous detection of Heme and Lactate.
To engineer a system where biosensing strains are coupled through a shared quorum-sensing signal.
To implement a gene circuit that computes the minimum between individual biosensor activity and the shared signal.

Main Methods:

Development of bacterial consortia with distinct biosensing strains.
Implementation of a global shared quorum-sensing signal to coordinate strain activity.
Design and evaluation of three consortia configurations: externally supplied signal, inducible gene circuit, and incoherent feedforward loop (IFFL).
Assessment of system performance and robustness against population perturbations.

Main Results:

Three consortia configurations were evaluated for Heme and Lactate detection.
The incoherent feedforward loop (IFFL) configuration demonstrated superior performance and robustness.
The IFFL system maintained low and stable shared signal levels, enhancing stability against population fluctuations.
The coupled consortia were successfully applied to monitor Heme and Lactate in humanized fecal samples.

Conclusions:

Bacterial consortia coupled via a shared quorum-sensing signal can function as sophisticated biosensors.
The IFFL gene circuit configuration offers an improved and robust method for coordinating multicellular biosensing.
This engineered system shows potential for diagnostic applications, specifically in monitoring Heme and Lactate in complex biological samples.