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Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
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Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
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Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
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Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
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Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...
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Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
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Updated: May 10, 2025

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Microfibril-Associated Protein 5 Contributes to the Elastic Fiber Abnormalities in Aged Skin.

Fumiaki Sato1, Teruaki Oku2, Yuka Nishigaki3

  • 1Laboratory of Pathological Analysis, Faculty of Pharmacy, Juntendo University, 6-8-1 Hinode, Urayasu, Chiba 279-0013, Japan.

Biological & Pharmaceutical Bulletin
|April 23, 2025
PubMed
Summary

Microfibril-associated protein 5 (MFAP5) excess in aging causes abnormal elastic fibers in skin. Reducing MFAP5 can potentially treat dermal aging and improve skin elasticity.

Keywords:
elastic fiberintrinsic agingmicrofibril-associated protein 5skin dermis

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Area of Science:

  • Dermatology
  • Cell Biology
  • Biochemistry

Background:

  • Elastic fibers provide tissue flexibility but are not regenerated after formation.
  • Degradation of elastic fibers contributes to aging and impacts tissue function.
  • Intrinsic aging causes dermal elastic fiber abnormalities, with underlying mechanisms unclear.

Purpose of the Study:

  • To identify factors responsible for intrinsic dermal aging-related elastic fiber abnormalities.
  • To investigate the role of microfibril-associated protein 5 (MFAP5) in these abnormalities.

Main Methods:

  • Comprehensive gene expression analysis to identify candidate genes.
  • Immunofluorescence staining to assess MFAP5 expression and localization in aged fibroblasts.
  • MFAP5 gene silencing and exogenous addition experiments.
  • Analysis of interactions between MFAP5, latent transforming growth factor β binding protein 4, and fibulin-5.

Main Results:

  • Aged fibroblasts showed high MFAP5 expression, localized to aggregated elastic fibers.
  • MFAP5 elimination suppressed elastic fiber aggregation.
  • Exogenous MFAP5 induced elastic fiber thickening and disorganization.
  • MFAP5 inhibited crucial protein interactions for elastic fiber formation.

Conclusions:

  • Excess MFAP5 expression in aging is a key driver of dermal elastic fiber abnormalities.
  • MFAP5 plays a significant role in the structural integrity of elastic fibers.
  • Targeting MFAP5 offers a potential therapeutic strategy for intrinsic dermal aging and skin elasticity improvement.