Terpenoids from Chloranthus holostegius with Anti-inflammatory Activities

Area of Science:

• Natural Products Chemistry
• Pharmacology

Background:

• Chloranthus holostegius is a plant species with potential medicinal properties.
• Terpenoids are a diverse class of natural products with various biological activities.

Purpose of the Study:

• To isolate and characterize new terpenoids from Chloranthus holostegius.
• To evaluate the anti-inflammatory potential of the isolated compounds.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structural elucidation using HR-ESI-MS, UV, IR, 1D/2D NMR, quantum chemical calculations (NMR/ECD), and X-ray crystallography.
• Anti-inflammatory activity assessment by measuring nitric oxide inhibition in LPS-stimulated RAW 264.7 cells.

Main Results:

• Five new terpenoids were isolated: one monoterpenoid, one diterpenoid, and three sesquiterpenoids.
• Compounds 1 (isocamphane-type monoterpenoid) and 5 (cadinane-type sesquiterpenoid) demonstrated moderate inhibitory activity against nitric oxide production.
• IC50 values for compounds 1 and 5 were 21.16 ± 1.37 µM and 18.03 ± 1.24 µM, respectively.

Conclusions:

• The study successfully identified novel terpenoids from Chloranthus holostegius.
• Compounds 1 and 5 possess anti-inflammatory properties, warranting further investigation.