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Related Experiment Video

Updated: May 10, 2025

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices

Published on: September 10, 2019

Innovative Molecular Imprinting Sensor for Quick, Non-Invasive Cortisol Monitoring in Fish Welfare.

Hugo G Santos^1,2,3, Daniela Santos Oliveira², Felismina T C Moreira²

¹CIIMAR-Interdisciplinary Centre for Marine and Environmental Research, University of Porto, 4460-314 Matosinhos, Portugal.

|April 25, 2025

Summary

A novel sensor for measuring fish stress hormone cortisol in water was developed. This affordable, non-invasive device uses a molecularly imprinted polymer on screen-printed electrodes for accurate fish welfare assessment.

Keywords:

European seabass aquaculture screen-printed carbon electrode (SPCE)welfare assessment zebrafish

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Last Updated: May 10, 2025

Cortisol Extraction from Sturgeon Fin and Jawbone Matrices

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Published on: September 10, 2019

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

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Continuous Noninvasive Measuring of Crayfish Cardiac and Behavioral Activities

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Published on: February 6, 2019

Area of Science:

Electrochemistry
Materials Science
Environmental Monitoring

Background:

Fish welfare is critical for aquaculture productivity and research integrity.
Current methods for assessing fish stress, like cortisol measurement, can be invasive or time-consuming.
There is a need for rapid, non-invasive tools to monitor fish welfare.

Purpose of the Study:

To develop an innovative electrochemical sensor for direct and selective detection of cortisol in fish farm water.
To create a cost-effective and user-friendly device for real-time fish welfare assessment.

Main Methods:

Fabrication of screen-printed carbon electrodes (SPCEs) functionalized with reduced graphene oxide/Prussian blue nanocubes (rGO/PBNCs).
Synthesis of a molecularly imprinted polymer (MIP) on rGO/PBNCs/SPCEs via electropolymerization of pyrrole in the presence of cortisol.
Characterization of electrode morphology and chemistry using SEM and Raman spectroscopy.

Main Results:

The developed sensor demonstrated selective detection of cortisol.
Optimized sensor exhibited a linear detection range from 0.1 nM to 0.1 mM in artificial saltwater.
The sensor combines simplicity, affordability, and reliability for detecting chemical and biological compounds.

Conclusions:

The novel MIP-based electrochemical sensor offers a promising solution for non-invasive fish welfare monitoring.
This technology can facilitate timely welfare evaluations and prompt corrective actions in aquaculture settings.
The sensor's design highlights the potential of functionalized SPCEs for sensitive analyte detection.