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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

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Biomimetic Prussian Blue Sensor for Ultrasensitive Direct Detection of Myoglobin.

Jacinta Ricardo¹, Abel Duarte¹, Stefano Chiussi²

¹CIETI-LabRISE, ISEP, Polytechnic of Porto, R. Dr. António Bernardino de Almeida, 431, 4249-015 Porto, Portugal.

|March 13, 2025

Summary

A new biosensor detects myoglobin (Myo) using Prussian Blue nanocubes and a biomimetic layer for point-of-care diagnostics. This cost-effective method offers high sensitivity and portability for rapid Myo analysis.

Keywords:

Prussian Blue nanocubes cardiac biomarker myoglobin redox-free liquid screen-printed electrodes surface molecular imprinting

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Published on: February 16, 2018

Area of Science:

Electrochemistry
Nanomaterials Science
Biomedical Engineering

Background:

Myoglobin (Myo) is a key biomarker for diagnosing cardiac conditions like myocardial infarction.
Current detection methods can be complex, time-consuming, and not suitable for point-of-care (PoC) settings.
There is a need for rapid, sensitive, and portable Myo detection systems for early diagnosis.

Purpose of the Study:

To develop a novel, cost-effective, and scalable biosensor for direct myoglobin detection.
To create a biomimetic sensing layer for enhanced specificity and sensitivity.
To validate the sensor's performance for potential PoC applications.

Main Methods:

Fabrication of a disposable platinum screen-printed electrode (Pt-SPE) modified with Prussian Blue nanocubes (PBNCs).
Electropolymerization of ortho-phenylenediamine (o-PD) in the presence of Myo to form molecularly imprinted polymer (MIP) sites.
Incubation with trypsin to create specific Myo binding cavities.
Analysis using cyclic voltammetry (CV) and differential pulse voltammetry (DPV).

Main Results:

The developed sensor demonstrated a linear response for Myo detection from 1.0 ag/mL to 10 ng/mL.
Achieved a highly sensitive limit of detection (LOD) of 0.76 ag/mL.
Exhibited excellent linearity with an R² value of 0.9775, indicating reliable quantification.

Conclusions:

The novel PBNCs/MIP/Pt-SPE biosensor offers a simplified, portable, and sensitive platform for direct Myo detection.
The absence of external redox probes enhances suitability for PoC applications.
This approach holds significant promise for rapid and accessible cardiac biomarker diagnostics.