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Promoting Dentin Bridge Formation Through N-Acetyl-L-Cysteine Application in Rat Molar Pulpotomy: An Experimental
Kota Takagi1, Koichi Nakamura1, Yoshitaka Yoshimura2
1Department of Dentistry for Children and Disabled Persons, Graduate School of Dental Medicine, Hokkaido University, Kita 13 Nishi 7, Kita-ku, Sapporo 060-8586, Japan.
Journal of Functional Biomaterials
|April 25, 2025
Summary
N-acetyl-L-cysteine (NAC) combined with mineral trioxide aggregate (MTA) significantly improved pulpotomy outcomes in rats by enhancing dentin bridge formation and reducing inflammation, suggesting MTA-NAC is a superior pulp capping agent.
Area of Science:
- Biomaterials Science
- Regenerative Dentistry
- Oral Biology
Background:
- Pulpotomy is a dental procedure to treat pulp exposure due to decay or trauma.
- Mineral trioxide aggregate (MTA) is a common pulp capping material, but its success rates can vary.
- N-acetyl-L-cysteine (NAC), an antioxidant, shows promise for improving regenerative outcomes.
Purpose of the Study:
- To evaluate the efficacy of MTA combined with NAC (MTA-NAC) compared to MTA alone for pulp capping in a rat model.
- To assess the impact of MTA-NAC on pulp tissue healing and dentin regeneration.
Main Methods:
- Pulpotomy was performed on Sprague Dawley rat maxillary molars.
- Pulp capping was done using Superbond C&B, MTA, or MTA-NAC.
- Histological analysis and assessment of dentin sialophosphoprotein (DSPP) and osteopontin expression were conducted at 3 and 7 days post-procedure.
Main Results:
- Both MTA and MTA-NAC reduced inflammation at 3 days.
- MTA-NAC promoted significant dentin bridge formation by 7 days.
- MTA-NAC resulted in more extensive DSPP and osteopontin expression compared to MTA alone.
Conclusions:
- NAC enhances the regenerative capacity of MTA in pulpotomy.
- MTA-NAC demonstrates superior performance in promoting dentin bridge formation and pulp healing.
- MTA-NAC represents a promising advancement for pulp capping procedures.
