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UBASH3A and TIGIT genes expression levels in systemic sclerosis.

Aml A R Mohammed1, Fatma S Abd-Elsamea2, Maha S I Abdelrahman3

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Systemic sclerosis patients show higher mRNA expression of UBASH3a and TIGIT. These immune regulatory genes may play a role in the autoimmune disease pathogenesis.

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Area of Science:

  • Immunology
  • Rheumatology
  • Molecular Biology

Background:

  • Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis and vasculopathy.
  • Proinflammatory and profibrotic cytokines like IL-4 and IL-13 are implicated in SSc pathogenesis.
  • UBASH3a and TIGIT are key regulators of T-cell activation and immune tolerance.

Purpose of the Study:

  • To investigate the messenger RNA (mRNA) expression levels of UBASH3a and TIGIT in patients with Systemic sclerosis.
  • To compare these expression levels with those in healthy control subjects.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were isolated from 30 SSc patients and 30 age/sex-matched controls.
  • Total RNA was extracted using commercial kits.
  • mRNA expression levels of UBASH3a and TIGIT were quantified using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR).

Main Results:

  • UBASH3a mRNA expression was significantly higher in SSc patients compared to controls.
  • TIGIT mRNA expression was also significantly elevated in SSc patients relative to controls.
  • These findings suggest altered expression of immune regulatory genes in SSc.

Conclusions:

  • The study demonstrates significantly higher mRNA expression of UBASH3a and TIGIT in PBMCs of Systemic sclerosis patients.
  • Elevated levels of UBASH3a and TIGIT may indicate a dysregulated immune response in SSc.
  • Further research is warranted to elucidate the specific roles of UBASH3a and TIGIT in SSc pathogenesis.