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UBASH3A and TIGIT genes expression levels in systemic sclerosis.
Aml A R Mohammed1, Fatma S Abd-Elsamea2, Maha S I Abdelrahman3
1Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt.
The Egyptian Journal of Immunology
|April 26, 2025
Summary
Systemic sclerosis patients show higher mRNA expression of UBASH3a and TIGIT. These immune regulatory genes may play a role in the autoimmune disease pathogenesis.
Area of Science:
- Immunology
- Rheumatology
- Molecular Biology
Background:
- Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis and vasculopathy.
- Proinflammatory and profibrotic cytokines like IL-4 and IL-13 are implicated in SSc pathogenesis.
- UBASH3a and TIGIT are key regulators of T-cell activation and immune tolerance.
Purpose of the Study:
- To investigate the messenger RNA (mRNA) expression levels of UBASH3a and TIGIT in patients with Systemic sclerosis.
- To compare these expression levels with those in healthy control subjects.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) were isolated from 30 SSc patients and 30 age/sex-matched controls.
- Total RNA was extracted using commercial kits.
- mRNA expression levels of UBASH3a and TIGIT were quantified using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR).
Main Results:
- UBASH3a mRNA expression was significantly higher in SSc patients compared to controls.
- TIGIT mRNA expression was also significantly elevated in SSc patients relative to controls.
- These findings suggest altered expression of immune regulatory genes in SSc.
Conclusions:
- The study demonstrates significantly higher mRNA expression of UBASH3a and TIGIT in PBMCs of Systemic sclerosis patients.
- Elevated levels of UBASH3a and TIGIT may indicate a dysregulated immune response in SSc.
- Further research is warranted to elucidate the specific roles of UBASH3a and TIGIT in SSc pathogenesis.
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