Antimicrobial peptide-ZIF8 embedded silk protein-lysozyme composite films: A promising multifunctional solution for
Xiaotong Shen1, Jiali Wang1, Bowen Cao1
1College of Lab Medicine, Life Science Research Centre, Key Laboratory of Biomedical Materials of Zhangjiakou, Hebei North University, Zhangjiakou 075000, China.
Abstract:
Bacterial infections as well as delayed osseointegration have always been clinical challenges for titanium-based orthopedic implants, and effective solutions are urgently needed. In this study, we cleverly utilized silk protein and lysozyme (SF-Lys) nanofilms as carriers, and coated metal-organic frameworks (ZIF8@P) encapsulated with antimicrobial peptides (P) onto titanium surfaces to construct a pH-responsive SF-Lys/ZIF8@P multifunctional coating. The coating demonstrated good biosafety, hemocompatibility, corrosion resistance, and hydrophilicity. Based on the synergistic effect of P with Zn2+, its inhibition rate of both E. coli and S. aureus reached >90 %. The coating was able to effectively induce RAW264.7 to M2-type polarization and remodel the anti-inflammatory microenvironment. In addition, the coating induced endothelial cell migration as well as lumen formation, accelerating the vascularization of HUVEC. Specifically, the coating was able to effectively differentiate MC3T3-E1 into osteoblasts by further stimulating the production of genes and proteins linked to osteogenesis. More importantly, the coating demonstrated excellent antibacterial, anti-inflammatory, vascularization and bone repair capabilities in animal models, which comprehensively solved the key problems faced by titanium implants in practical applications. In conclusion, the SF-Lys/ZIF8@P coating provides a comprehensive and promising solution for infected bone repair, with significant clinical prospects.
