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Late hexokinase activity expression during Bufo bufo development.

M Miranda

    Biochimica Et Biophysica Acta
    |March 29, 1977
    PubMed
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    Hexokinase activity appears late in Bufo bufo embryo development, after a significant rise in glucose. This suggests glucose alone does not trigger hexokinase expression in vivo.

    Area of Science:

    • Developmental Biology
    • Biochemistry
    • Amphibian Embryology

    Background:

    • Hexokinase is a key enzyme in glucose metabolism.
    • Understanding hexokinase regulation is crucial for studying embryonic development and energy utilization.
    • Previous studies noted low glucose utilization in early amphibian development.

    Purpose of the Study:

    • To investigate the temporal expression of hexokinase during Bufo bufo embryonic development.
    • To determine the relationship between free glucose levels and hexokinase activity in Bufo bufo embryos.
    • To identify potential inducers of hexokinase activity in vivo.

    Main Methods:

    • Analysis of hexokinase activity in cytosols and homogenates from various developmental stages of Bufo bufo embryos.
    • Measurement of free glucose concentrations in embryo cytosols across different developmental stages.

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    Main Results:

    • Hexokinase activity was detected from stage 17 onwards in Bufo bufo embryos.
    • Free glucose was present in embryo cytosols at all tested stages.
    • A significant increase in cytosol glucose content occurred at stage 15, preceding hexokinase expression.

    Conclusions:

    • Hexokinase expression in Bufo bufo embryos is not directly induced by glucose alone.
    • The late onset of hexokinase expression may explain the observed low glucose utilization in early amphibian development.
    • Further research is needed to elucidate the specific inducers of hexokinase in vivo.