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Updated: May 9, 2025

Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
Balancing stability and function: impact of the surface charge of SARS-CoV-2 Omicron spike protein
Daniel Lauster1, Rainer Haag2, Matthias Ballauff2
1Institute of Pharmacy, Biopharmaceuticals, Freie Universität Berlin, Berlin, Germany. daniel.lauster@fu-berlin.de.
Abstract:
The ectodomain of the Omicron SARS-CoV-2 spike has an increased positive surface charge, favoring binding to the host cell surface, but may affect the stability of the ectodomain. Thermal stability studies identified two transitions associated with the flexibility of the receptor binding domain and the unfolding of the whole ectodomain, respectively. Despite destabilizing effects of some mutations, compensatory mutations maintain ECD stability and functional advantages thus supporting viral fitness.
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