Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Glimpse into the Initial Microsecond of Biomolecular Condensation.

Journal of the American Chemical Society·2026

Same author

Cellular water-potential sensing through biomolecular condensation.

Nature·2026

Same author

Distinct RNA Physical Microenvironments Shape Unique Properties and Functions within Biomolecular Condensates.

Journal of the American Chemical Society·2026

Same author

Ambroxol hydrochloride and clenbuterol hydrochloride oral solution for wheezing disorders in children in China: evidence mapping and meta-analysis.

Frontiers in pediatrics·2026

Same author

Bridging hydrogen-bond-stabilized polymeric coacervate core micelles for high-efficiency drug encapsulation and delivery.

Nature communications·2026

Same author

Incorporating meteorological factors into a SARIMA model for predicting pediatric influenza epidemics.

Frontiers in public health·2026

Search research articles

Related Experiment Video

Updated: May 14, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Exploring Endoplasmic Reticulum Dysfunction on Protein Phase Separation Using Viscosity-Sensitive Fluorescent

Yu Wei¹, Xiangyu Zi¹, Jia Zhai¹

¹School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210023, China.

Analytical Chemistry

|April 29, 2025

Summary

New probes visualize protein phase transitions linked to degenerative diseases. Researchers found calcium ions (Ca2+) regulate TDP-43 aggregation, offering potential therapeutic insights.

More Related Videos

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

Related Experiment Videos

Last Updated: May 14, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

Area of Science:

Biochemistry and Molecular Biology
Cellular Biology
Neuroscience

Background:

Protein phase transitions are implicated in degenerative diseases.
Endoplasmic reticulum (ER) homeostasis imbalance triggers these transitions.
Monitoring ER microenvironment changes is vital for studying protein phase behavior.

Purpose of the Study:

Develop novel viscosity-sensitive probes for monitoring ER microenvironment.
Investigate the role of ER stress and Ca2+ signaling in TDP-43 protein phase separation.
Provide tools for understanding degenerative disease mechanisms.

Main Methods:

Developed dicyanomethylene-4H-pyran (VisDCM) based viscosity-sensitive fluorescent probes.
Utilized computational analysis to understand probe fluorescence activation mechanisms.
Designed dual-color probes for simultaneous ER and protein visualization.
Performed in vitro experiments and fluorescence lifetime imaging.

Main Results:

VisDCM probes exhibit dual fluorescence response to viscosity, activated by restricted accessible conical intersection.
ER stress regulates TDP-43 protein phase separation via Ca2+ signaling.
Increased Ca2+ promotes TDP-43 liquid-liquid phase separation and aggregation.
Fluorescence lifetime imaging successfully mapped ER stress-induced microenvironment changes.

Conclusions:

VisDCM probes offer a novel toolbox for visualizing protein phase transitions.
Ca2+ plays a critical role in TDP-43 phase separation and aggregation.
Findings provide insights into degenerative diseases and potential therapeutic strategies.