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Suspended Tissue Open Microfluidic Patterning (STOMP).

Amanda J Haack1,2, Lauren G Brown1, Alex J Goldstein3,4,5

  • 1Department of Chemistry, University of Washington, Seattle, WA, 98195, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|April 29, 2025
PubMed
Summary
This summary is machine-generated.

Scientists developed Suspended Tissue Open Microfluidic Patterning (STOMP) to create multi-regional suspended tissues. This method enables studying complex tissue interfaces and reveals altered cell contraction dynamics at these boundaries.

Keywords:
hydrogel patterningopen microfluidicssuspended tissuetissue engineering

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Area of Science:

  • Mechanobiology
  • Biomaterials Engineering
  • Tissue Engineering

Background:

  • Free-standing suspended tissues are valuable tools for studying cell contraction.
  • Existing models typically consist of single-region tissues, limiting the study of complex tissue interfaces.
  • Natural tissues often feature heterogeneous regions, such as diseased-healthy or tissue-type boundaries.

Purpose of the Study:

  • To develop a novel method for creating multi-regional suspended tissues.
  • To enable the study of complex tissue interfaces and their impact on cellular dynamics.
  • To provide a versatile platform for generating engineered tissues with controlled spatial patterning.

Main Methods:

  • Suspended Tissue Open Microfluidic Patterning (STOMP) utilizes open microfluidics and capillary pinning.
  • This technique patterns subregions within free-standing engineered tissues.
  • The method is compatible with native extracellular matrices and advanced 4D materials.

Main Results:

  • STOMP successfully created multi-regional suspended tissues, including fibrotic-healthy engineered heart tissues and bone-ligament enthesis constructs.
  • Altered contractile dynamics were observed in fibrotic-healthy tissues compared to single-region controls.
  • Differential contractility was noted in bone-ligament enthesis constructs versus single-tissue periodontal ligament models.

Conclusions:

  • STOMP is a versatile platform for generating patterned, free-standing tissues.
  • The method facilitates the study of cellular behavior at complex tissue interfaces.
  • STOMP combines the contractile functionality of suspended tissues with precise spatial control for advanced mechanobiology studies.