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Finger-stick Blood Sampling Methodology for the Determination of Exercise-induced Lymphocyte Apoptosis
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Aerobic Exercise Affected Lymphocyte Apoptosis by Modulating ROS Release and NLRP3 Inflammasome Activation.

X Lian1,2, Z Guo1,2, J Liu3

  • 1Rehabilitation Assessment Department, Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China.

Bulletin of Experimental Biology and Medicine
|April 29, 2025
PubMed
Summary

High-intensity exercise induces splenic lymphocyte apoptosis via oxidative stress and NLRP3 inflammasome activation. Moderate exercise, however, does not significantly alter these immune parameters.

Keywords:
NLRP3 inflammasomeaerobic exerciseapoptosis of lymphocytesimmune functionreactive oxygen species (ROS)

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Area of Science:

  • Immunology
  • Exercise Physiology
  • Cell Biology

Background:

  • Moderate exercise enhances immune function, but high-intensity exercise can impair it.
  • The mechanisms by which physical load affects splenic lymphocyte apoptosis are not fully understood.

Purpose of the Study:

  • To investigate the impact of exercise intensity on splenic lymphocyte apoptosis in rats.
  • To explore the underlying mechanisms, including oxidative stress and inflammasome activation.

Main Methods:

  • Developed a physical exercise model in rats.
  • Analyzed splenic lymphocyte apoptosis markers, oxidative stress (ROS), mitochondrial membrane potential, and NLRP3 inflammasome activation.

Main Results:

  • High-intensity exercise decreased the CD4+/CD8+ ratio, increased oxidative stress, mitochondrial membrane potential, and activated NLRP3 inflammasomes.
  • High-intensity exercise promoted pro-apoptotic proteins and decreased anti-apoptotic proteins.
  • Moderate exercise showed no significant changes in ROS or mitochondrial membrane potential and opposite effects on other parameters compared to high-intensity exercise.

Conclusions:

  • High-intensity exercise promotes splenic lymphocyte apoptosis through excessive reactive oxygen species (ROS) release and NLRP3 inflammasome activation.
  • Exercise intensity critically influences immune responses within the spleen.