Related Experiment Video
Updated: Jun 13, 2025
Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
Published on: February 23, 2014
Clinical features and antibiotic resistance in pediatric pneumococcal meningitis in Southern Vietnam, 2012-2023: A
Hieu Cong Truong1, Thanh Van Phan2, Hung Thanh Nguyen3
1Department for Disease Control and Prevention, Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City, Vietnam; Institute of Health and Society, Université catholique de Louvain, Brussels, Belgium.
Background:
Streptococcus pneumoniae is a major cause of morbidity and mortality in children. This study investigates the clinical features, cerebrospinal fluid (CSF) findings, serotype distribution, and antibiotic resistance patterns in Vietnamese children aged 1-59 months with pneumococcal meningitis (PM).
Methods:
This retrospective study (2012-2023) was conducted at two tertiary pediatric hospitals in Ho Chi Minh City. CSF samples from probable bacterial meningitis (PBM) cases were analyzed using biochemistry, culture, and real-time polymerase chain reaction (rt-PCR). Serotyping and antibiotic-resistance genes were identified using quadriplex rt-PCR.
Results:
Among 2922 PBM cases, 155 (5.3 %) were confirmed as PM. Of these, 58.7 % occurred in children under one year and 62.6 % during the rainy season. Fever (98.1 %) and vomiting (67.7 %) were the most common symptoms. Infants under 12 months frequently exhibited nonspecific signs like convulsions (48.4 %) and bulging fontanels (34.1 %), while older children displayed classic symptoms such as neck stiffness (32.4 %) and behavioral changes (26.5 %). CSF analysis revealed turbid appearance, WBC ≥ 100 cells/mm³ (85.2 %), and protein ≥ 1 g/L (60.0 %) (p < 0.05). Severe outcomes were noted in 17.4 % of PM cases, with a 3.2 % fatality rate. The 13-valent pneumococcal conjugate vaccine (PCV13) serotypes caused 81.3 % of confirmed cases, predominantly serotypes 6 A/B (34.8 %) and 19 F (20.0 %). Among 137 isolates tested, high prevalence rates were observed for the pbp2b (68.6 %), mef(A)/erm(B) (93.4 %), and tetM (92.0 %) resistance genes. Additionally, 61.3 % of isolates showed multiple resistance genes, particularly in serotypes 6 A/B, 23 F, 9 V, and 13. Antibiotic resistance in non-PCV13 serotypes increased over time.
Conclusions:
PM in Vietnamese children presents age-specific clinical presentations and is predominantly caused by highly resistant PCV13 serotypes. The rising resistance in non-PCV13 serotypes poses a formidable challenge in managing pneumococcal infections. These findings emphasize the urgent need for PCV introduction in the national immunization program and ongoing resistance surveillance.
More Related Videos
06:06Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43
Published on: September 11, 2020
12:21A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness
Published on: September 28, 2022
Related Concept Videos
01:30Pneumonia III: Complications and Assessment
01:28Pneumonia IV: Management
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
01:30Pneumonia I: Introduction
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...