Evaluating the quality consistency of Nuangong Qiwei Pills based on integrated HPLC, DSC and electrochemistry...

Nuangong Qiwei Pill (NQP) is a classic Mongolian formula consisting of seven herbs, which is widely used in the treatment of gynecological diseases. To provide an integrated and comprehensive quality assessment method for NQP, multidimensional fingerprint was creatively applied to NQP. Firstly, the contents of three compounds-eugenol, agarotetrol and dehydrodiisoeugenol in 18 batches of NQPs were quantitatively analyzed. Then, the multidimensional fingerprints were established, which included the establishment of three-wavelength fusion HPLC fingerprints and their reliability, the obtaining of electrochemical fingerprints by B-Z oscillation and the analysis of nine parameters, and the obtaining of differential scanning calorimetry (DSC) quantum fingerprints by fixed-point merger method. Subsequently, systematically quantitative fingerprint method (SQFM) was employed to evaluate similarities, Finally, the qualitative and quantitative results of the fingerprint were synthesized using the coefficient of variation weighting method (CVWM), and the samples were classified into 1-5 levels, which satisfied the evaluation criteria (S_m ≥ 0.70, 70 % ≤ P_m ≤ 130 %). Overall, this study provides valuable information for ensuring the quality of NQPs and will promote the application of fingerprint technology in the Mongolian medicine filed.