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MINFLUX nanoscopy enhanced with high-order vortex beams.

Xiao-Jie Tan1, Zhiwei Huang2,3,4

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Summary

High-order vortex beams significantly boost Minimal Photon Fluxes (MINFLUX) nanoscopy performance. This advancement offers improved localization precision and a wider field of view for super-resolution imaging.

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Area of Science:

  • Optical microscopy
  • Super-resolution imaging
  • Nanoscale science

Background:

  • Minimal Photon Fluxes (MINFLUX) nanoscopy achieves super-resolution by precisely localizing fluorescent molecules.
  • Conventional MINFLUX typically utilizes first-order vortex beams for optical manipulation.

Purpose of the Study:

  • To investigate the potential of high-order vortex beams for enhancing MINFLUX performance.
  • To theoretically analyze the impact of beam order on localization precision and field of view.

Main Methods:

  • Theoretical analysis of MINFLUX principles.
  • Modeling the interaction of high-order vortex beams with fluorescent emitters.
  • Simulating standard and raster scan MINFLUX configurations.

Main Results:

  • High-order vortex beams can improve MINFLUX localization precision by a factor related to their order.
  • Sub-nanometer localization precision is achievable under optimal conditions.
  • Raster scan MINFLUX with high-order vortex beams offers a wider field of view with maintained precision.

Conclusions:

  • High-order vortex beams represent a significant advancement for MINFLUX nanoscopy.
  • This approach promises to push the boundaries of super-resolution imaging.
  • Potential for broader applications in ultra-high resolution biological imaging.