Related Experiment Video
Updated: May 9, 2025

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Infection Risk Following Contaminated Cases.
Sean Baran1, Rishikesan Ramaesh1, Alexander Y Shin1
1Orthopedic Department, Mayo Clinic, Rochester, Minnesota.
Performing clean orthopedic surgery after a dirty procedure in the same operating room did not increase infection risk. Bacterial analysis showed no direct cross-contamination, suggesting current protocols are safe for preventing surgical site infections.
Area of Science:
- Orthopedic Surgery
- Infectious Disease Control
- Operating Room Protocols
Background:
- Concerns exist regarding the safety of performing clean surgical procedures immediately after contaminated (type IV) procedures in the same operating room.
- The risk of infection transmission in sequential surgeries remains a critical issue in healthcare settings.
Purpose of the Study:
- To investigate the infection rates in clean orthopedic surgical wounds (type I) performed immediately after contaminated wounds (type IV).
- To determine if direct bacterial cross-contamination occurs between sequential 'dirty' and 'clean' surgical cases.
Main Methods:
- A retrospective review of orthopedic surgical cases between 2003 and 2010.
- Identified 674 instances where a type I wound procedure followed a type IV wound procedure.
- Analyzed the incidence of surgical site infections (SSIs) in type I cases and compared bacterial profiles.
Main Results:
- 3.3% of clean (type I) surgical wounds developed SSIs after being performed subsequent to a contaminated (type IV) wound.
- In no case was the bacterial profile of the SSI in the type I case identical to the preceding type IV case.
- This indicates that direct cross-contamination was not the source of infection in these instances.
Conclusions:
- Performing clean orthopedic surgery immediately after a contaminated procedure in the same operating room does not appear to significantly increase SSI risk.
- Direct bacterial cross-contamination is unlikely to be the primary cause of infections in clean cases following dirty cases.
- Findings support the current practice of sequential surgeries, provided appropriate sterilization protocols are maintained.
Related Concept Videos
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Infection
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
Transmission-based Precautions I: Contact, Enteric, and Droplets
Contact Precautions:
Contact precautions are the measures taken to prevent the transmission of infectious agents, especially epidemiologically important microorganisms such as MRSA or influenza, primarily transmitted through direct or indirect contact with an...
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Factors Affecting the Risk of Infection
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...

