Current Development of iPSC-Based Modeling in Neurodegenerative Diseases

Area of Science:

• Neuroscience
• Stem Cell Biology
• Genetics

Background:

• Induced pluripotent stem cell (iPSC) technology has seen significant progress over the last 20 years.
• These advancements have expanded the use of iPSCs in neuroscience, enhancing disease understanding and therapeutic target investigation.
• Optimized reprogramming and neuronal differentiation techniques have improved the generation of iPSC-derived neural cells.

Purpose of the Study:

• To review the development and application of iPSC-based models in neurodegenerative diseases.
• To highlight the role of iPSCs in understanding disease pathogenesis and identifying therapeutic strategies.
• To discuss the integration of cerebral organoids and CRISPR/Cas9 genome editing in neurodegenerative disease research.

Main Methods:

• Review of scientific literature on iPSC technology and its applications in neurodegenerative diseases.
• Focus on advancements in reprogramming, neuronal differentiation, and maturation techniques.
• Integration of cerebral organoid technology and CRISPR/Cas9 genome editing for disease modeling.

Main Results:

• iPSC technology has become a powerful tool for modeling neurodegenerative diseases.
• Patient-derived and CRISPR-edited iPSCs and cerebral organoids serve as valuable disease models.
• These models contribute to understanding disease pathophysiology and identifying novel therapeutic targets.

Conclusions:

• iPSC-based models, particularly cerebral organoids, represent a significant advancement in studying neurodegenerative diseases.
• The integration of genome editing further enhances their utility for disease research and therapeutic development.
• These models are critical for advancing our knowledge of Alzheimer's, Parkinson's, and Huntington's diseases.