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Updated: May 12, 2025

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments
Published on: June 27, 2022
Novel Focused Ion Beam Techniques for Enhanced Sample Preparation for In Situ Transmission Electron Microscopy
Kerui Wei1, Matthew Lindley2, Xuzhao Liu2
1Henry Royce Institute, University of Manchester, Oxford Road, Manchester M13 9PL, UK.
Abstract:
Focused ion beam (FIB) systems have revolutionized sample preparation for transmission electron microscopy (TEM), enabling precise and site-specific material analysis. However, the conventional ion beam-induced deposition (IBID) approach to preparing FIB samples can lead to contamination effects that can compromise the quality of TEM data acquisition. This study introduces an innovative FIB method for connecting TEM lamellae to support grids via redeposition, avoiding the contamination issue. We demonstrate the effectiveness of this technique through observations of a SiC phase within tristructural-isotropic particles during in situ high-temperature and irradiation TEM experiments, establishing an improved process for characterizing material behaviors during exposure to their industrially relevant environmental conditions.
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