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Updated: May 12, 2025

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
Reversible Inhibition of Cysteine Proteases Using S-Methyl Methanethiosulfonate
Srđan Marković, Natalija Polović1
1Department of Biochemistry, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia. polovicn@chem.bg.ac.rs.
Abstract:
Cysteine proteases find applications in diverse fields, but their susceptibility to autolysis poses storage challenges. Unlike irreversible inhibitors, S-methyl methanethiosulfonate (MMTS) enables enzyme reactivation, offering an ideal solution for preventing autolysis during storage and allowing subsequent protein activation when required. Here, we describe effective methods for inhibiting and reactivating papain, providing valuable insights for enhancing the storage and usability of cysteine proteases in various scientific, medical, and industrial applications.
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