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Updated: May 13, 2025

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Advances in Chromatography-Free and Ambient Ionization Mass Spectrometry Techniques Toward Clinical Toxicology
William J Feeney1, Ibrahim Choucair1
1Department of Pathology and Laboratory Medicine, University of Kentucky College of Medicine, 800 Rose street, HA610, Lexington, KY 40536, USA.
Abstract:
This review presents current scientific literature focused on chromatography-free/ambient ionization mass spectrometry techniques for clinical toxicology. Traditional methods like gas and liquid chromatography, paired with mass spectrometry, are commonly used in clinical laboratories but can be time-consuming, leading to backlogs. To alleviate workloads, immunoassays are often used for screening before confirmatory methods but are susceptible to false positives/negatives especially with structurally similar compounds. Therefore, chromatography-free ionization mass spectrometry methods could address concerns regarding uncertainty and lengthy separation. This work examines current efforts implementing these techniques along with their capabilities, limitations, and potential for future integration into clinical toxicology.
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