Combined Effect of Low-level Laser Therapy and Hyaluronic Acid Injection in Oral Wound Healing: An Experimental Study

This study aimed to evaluate the effect of low-level laser therapy and hyaluronic acid injection in intraoral wound healing. Eighteen Wister albino rats were included in this study, and they were randomly divided into 3 groups; in the first group, a wound was created with a 450-nm diode laser in the buccal mucosa without any further intervention, and in the second group, the wound was created with a 450-nm diode laser followed by non-cross-linked hyaluronic acid injection. In the third group, the wound was created with a 450-nm diode laser followed by low-level laser therapy with a 650-nm diode laser and hyaluronic acid injection. Biopsies were taken at different intervals (baseline, third day, and seventh day) for evaluation of the wound healing using hematoxylin stain, Masson trichrome stain, and immunohistochemical study for CD34 analysis. Group 3 showed significant results compared with the other groups on the third and seventh postoperative days, with increased collagen production and better tissue organization with abundant angiogenesis. Low-level laser therapy in addition to hyaluronic acid injection in incisions created with lasers shows promising results in wound healing and may quicken the healing process.