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Published on: November 30, 2022
Fluorescent probes for lysosomes, mitochondria, and lipid droplets: precision design, dynamic microenvironment
Wen-Wen Xiao1, Shuai Chen1,2, Meng-Xian Liu1
1Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. chenshuai@mail.neu.edu.cn.
Abstract:
Organelles are essential for regulating cellular physiological processes and maintaining homeostasis. Disruption of their functions can lead to cellular dysfunction and contribute to various diseases. Advances in fluorescent materials and imaging technologies have significantly enhanced the development of probes for detecting organelle-specific parameters and studying their heterogeneity. This review summarizes the design strategies, response mechanisms, and applications of fluorescent probes targeting three key organelles - lysosomes, mitochondria, and lipid droplets - in microenvironmental sensing and heterogeneity analysis, as developed by our group and others. In addition, the challenges faced by organelle imaging and the outlook for future development are also discussed, aiming to inspire further innovation in the design and application of organelle-specific fluorescent probes.
