Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 21, 2025

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

A Preprocessing Toolbox for 2-Photon Subcellular Calcium Imaging.

Anqi Jiang¹, Chong Zhao^2,3, Mark E J Sheffield⁴

¹Departments of Neurobiology, Neuroscience Institute and.

|May 13, 2025

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Malignant transformation risk and management dilemmas of atypical lobular endocervical glandular hyperplasia: A retrospective cohort study comparing Peutz-Jeghers syndrome versus sporadic cases.

Gynecologic oncology·2026

Same author

GTPBP4 promotes colorectal cancer cell proliferation by positively regulating MYC-driven glycolytic metabolism.

American journal of cancer research·2026

Same author

The MAPK Pathway Coordinates an Immunosuppressive Microenvironment in Colorectal Cancer: A Single-Cell Guided Prognostic Model.

Cancer informatics·2026

Same author

FKBP9 Enhances Colon Cancer Cell Proliferation by Inhibiting GPX4-Mediated Ferroptosis.

Cancer medicine·2026

Same author

Global burden, projections, and causal factors of maternal sepsis and other maternal infections: A comprehensive epidemiological and mendelian randomization study.

PLoS neglected tropical diseases·2026

Same author

Distinct CA3 inputs differentially shape the learning-dependent evolution of right CA1 spatial maps.

Nature communications·2026

Same journal

Ocular speech tracking persists in blindness, but its dynamics and oculo-cerebral connectivity depend on visual status.

eNeuro·2026

Same journal

Emergent multidien cycles from partial circadian synchrony.

eNeuro·2026

Same journal

Adolescent social isolation induces persistent impairments in emotional discrimination and helping behavior.

eNeuro·2026

Same journal

Increased Ih Current Is Associated with Reduced Hippocampal CA1 Excitability in a Mouse Model of Multiple Sclerosis.

eNeuro·2026

Same journal

Reduced SuM Activation Accompanies Impaired Social Novelty Recognition in Mouse Models of Neurodevelopmental Disorders.

eNeuro·2026

Same journal

Do Not Forget the Stimulus: A Missing Control in Naturalistic Studies of Neural Entrainment.

eNeuro·2026

See all related articles

This study introduces a new computational pipeline to improve the analysis of two-photon calcium imaging data from neuronal subcellular compartments during mouse behavior. The method enhances signal detection, removes motion artifacts, and groups regions of interest for clearer physiological signal extraction.

Area of Science:

Neuroscience
Computational Biology
Biophysics

Background:

Two-photon calcium imaging allows recording neuronal activity from subcellular compartments like axons and dendrites during behavior.
Challenges include low signal-to-noise, inaccurate region-of-interest (ROI) identification, motion artifacts, and grouping ROIs from the same neuron.

Purpose of the Study:

To develop a computationally efficient preprocessing pipeline for subcellular signal detection, movement artifact identification, and ROI grouping.
To standardize the extraction of physiological signals from subcellular compartments during rodent behavior using two-photon calcium imaging.

Main Methods:

Subcellular signal detection using Fast Fourier Transform (FFT) and bandpass filtering (0.05-0.12 Hz) on calcium traces.
Movement artifact removal via Principal Component Analysis (PCA) and change-point detection on the first principal component.

Keywords:

2-photon axons calcium imaging dendrites subcellular

More Related Videos

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Related Experiment Videos

Last Updated: May 21, 2025

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Published on: February 18, 2020

Calcium transient identification by analyzing prominence and duration; ROI grouping using hierarchical or k-means clustering.

Main Results:

The pipeline effectively detects subcellular signals and removes motion artifacts.
Clustering methods accurately group active ROIs, yielding results comparable to ground truth data.
Demonstrated effectiveness using axon ROIs in the CA1 region of the mouse brain.

Conclusions:

The proposed pipeline offers a standardized approach for analyzing subcellular neuronal activity from two-photon calcium imaging data.
This method addresses key challenges, improving the reliability and interpretability of neurophysiological recordings during behavior.