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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Updated: May 2, 2026

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Tri-Mode CRISPR-Based Biosensor for miRNA Detection: Enhancing Clinical Diagnostics with Cross-Validation.

Jiangbo Dong¹, Wenhao Ma¹, Shiying Zhou¹

¹Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, P. R. China.

Analytical Chemistry

|May 13, 2025

Summary

We developed a CRISPR/Cas12a-powered trimode biosensor for ultrasensitive microRNA detection. This novel platform offers reliable, rapid, and cross-validated results for potential early cancer diagnosis.

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Area of Science:

Biotechnology
Molecular Diagnostics
Nanomaterials

Background:

Accurate detection of ultralow concentration disease biomarkers is crucial for in vitro diagnostics.
Multimode sensing offers enhanced reliability through data cross-validation and self-calibration.

Purpose of the Study:

To develop a CRISPR/Cas12a-powered trimode biosensor for ultrasensitive and reliable microRNA (miRNA) analysis.
To achieve enhanced diagnostic accuracy through multimode detection and cross-validation.

Main Methods:

Utilized a CRISPR/Cas12a system combined with rolling circle extension-driven loop-mediated isothermal amplification (R-LAMP) for signal amplification.
Employed methylene blue (MB) released from Fe-MOF nanocarriers as a signal probe.
Integrated electrochemical (EC), fluorescence (FL), and ultraviolet-visible (UV-vis) spectroscopy for trimodal detection.

Main Results:

Achieved rapid detection of target miRNA at femtomolar levels within 70 minutes.
Demonstrated enhanced reliability through cross-validation across the three sensing modes.
Successfully applied the biosensor to real samples, showing good agreement with RT-qPCR.

Conclusions:

The CRISPR/Cas12a-powered trimode biosensor provides ultrasensitive and reliable miRNA detection.
The platform's ability to cross-validate results enhances diagnostic accuracy.
This biosensor shows significant potential for the clinical diagnosis of early-stage cancer.