Validation of UV-Vis spectrophotometric colorimetric methods for Rifampicin quantification in PBS & biological...

Area of Science:

• Pharmacology and Drug Delivery
• Analytical Chemistry
• Neuroscience

Background:

• Tuberculous meningitis (TBM) treatment with rifampicin (RIF) is limited by poor blood-brain barrier penetration.
• Smart-pH nanoparticles and thermosensitive gels enhance RIF delivery to the inflamed brain.
• Olfactory nerve pathway is explored for targeted drug delivery in TBM.

Purpose of the Study:

• To develop and validate a robust analytical method for quantifying rifampicin (RIF).
• To enable accurate measurement of RIF in various biological matrices for drug delivery studies.
• To support the development of novel RIF delivery systems for tuberculous meningitis (TBM).

Main Methods:

• UV-Vis spectrophotometry and colorimetric assays were employed for RIF quantification.
• Method validation included selectivity, linearity, LOD, LOQ, accuracy, and precision assessments.
• Analysis was performed in phosphate-buffered saline (PBS), plasma, and brain tissue.

Main Results:

• The method demonstrated excellent linearity (r² = 0.999) across all tested media.
• Low limits of detection (LOD) between 0.25-0.49 µg/mL were achieved.
• High accuracy (-11.62% to 14.88% RE) and precision (2.06% to 13.29% RSD) met regulatory standards.

Conclusions:

• A validated analytical method provides reliable RIF quantification in biological samples.
• This method is crucial for in vitro, ex vivo, and in vivo drug delivery studies.
• Findings advance targeted drug delivery strategies for improved TBM treatment outcomes.