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Transcription factor OsNAC29a confers drought tolerance through the ABA pathway in rice
Jia Lu1, Weiting Wang1, Siqi Yang1
1College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
Abstract:
The plant-specific NAC transcription factor family plays a crucial role in mediating responses to abiotic stress, but the functions of many NAC genes remain poorly characterized. The rice OsNAC29a gene is induced by PEG and abscisic acid (ABA). OsNAC29a exhibits transactivation activity and the region of 248-315 amino acids at its C-terminus is essential for its activation. Over-expression of OsNAC29a enhances drought resistance and ABA sensitivity in transgenic rice. OsNAC29a over-expression modulates physiological indicators related to stress resistance, while RNAi-mediated down-regulation of OsNAC29a results in opposite phenotypes and physiological changes. Under drought conditions, OsNAC29a over-expression significantly up-regulates stress-related genes such as OsP5CS1, OsSRO1c, OsPOD1, OsLEA3, and OsRab16C. Interestingly, OsPOD1 gene expression increases in OsNAC29a over-expression rice under both normal and drought stress conditions, leading to significantly enhanced peroxidase activity. Further research reveals that OsNAC29a binds to the OsPOD1 promoter to drive its expression. Additionally, OsSAPK2 which is a key component of the ABA-dependent drought-tolerance pathway interacts physically with OsNAC29a and enhances its transcriptional activation activity. Collectively, acting as a positive regulator of drought tolerance, OsNAC29a regulates drought resistance in rice by directly or indirectly modulating stress-responsive genes.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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