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VRK1/BANF1/GLI1 Axis Regulates Tumor Development and Progression of Colorectal Cancer.
Xu Wang1, Yuanmin Xu1, Shixin Chan1
1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui, China.
International Journal of Biological Sciences
|May 19, 2025
Summary
Barrier to Autointegration Factor 1 (BANF1) is elevated in colorectal cancer (CRC) and drives tumor progression by regulating GLI1. Targeting the VRK1/BANF1/GLI1 pathway offers a potential therapeutic strategy for advanced CRC.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Barrier to Autointegration Factor 1 (BANF1) association with diseases is known, but its role in colorectal cancer (CRC) is unexplored.
- Understanding BANF1's mechanism in CRC is crucial for developing new therapeutic strategies.
Purpose of the Study:
- To investigate the role and mechanism of BANF1 in colorectal cancer (CRC) initiation and progression.
- To identify potential therapeutic targets within the BANF1 regulatory pathway in CRC.
Main Methods:
- BANF1 expression analysis in CRC tissues and cell lines using bioinformatics, PCR, Western Blot, and IHC.
- In vitro and in vivo experiments to assess BANF1's functional role in CRC.
- RNA sequencing and bioinformatics to identify regulatory pathways and interacting proteins (VRK1).
Main Results:
- BANF1 expression is significantly upregulated in CRC cells and tissues, serving as an independent prognostic factor.
- BANF1 promotes CRC phenotypes by regulating GLI1 expression.
- Vaccinia-related kinase 1 (VRK1) acts as an upstream regulator, interacting with BANF1 to influence CRC progression.
Conclusions:
- The VRK1/BANF1/GLI1 axis is a key molecular pathway in colorectal cancer.
- Targeting the VRK1/BANF1/GLI1 axis presents a promising therapeutic strategy for advanced CRC.
- This research provides novel insights into CRC pathogenesis and potential treatment avenues.
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