Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

205.2K
Overview
205.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.1K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.1K
From DNA to Protein03:06

From DNA to Protein

18.1K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
18.1K
DNA Isolation01:24

DNA Isolation

38.5K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
38.5K
Next-generation Sequencing03:00

Next-generation Sequencing

88.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.5K
Sanger Sequencing01:57

Sanger Sequencing

753.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
753.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ratiometric Fluorescence Biosensor of Allosteric Ag Nanoclusters by G-Quadruplex-Aided Displacement Amplification.

ACS sensors·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Unlocking Self-Luminescence of Pyrene-Based Metal-Organic Gel for Sensitive Electrochemiluminescence Assay of Microplastics.

Analytical chemistry·2026
Same author

Bivariate Cooperator-Catalyzed Hairpin Assembly for Amplifying Conformation-Guided Label-Free Ratiometric Fluorescence Biosensing.

Analytical chemistry·2026
Same author

Ordered DNA Cube-Braced Hierarchical Ladder Track-Confined Efficient Circular DNA Walker for Rapid and Ultrasensitive Electrochemical Detection of UDG Activity.

Analytical chemistry·2026
Same author

A Synergistic DNA Walker-Track System with Tailored Spatial Compatibility for Rapid and Ultrasensitive Electrochemical Detection of MUC1.

Analytical chemistry·2026

Related Experiment Video

Updated: Jun 16, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K

A Programmed DNA Dynamic Assembly-Guided Molecular Amplifier for Authentic Information Decryption.

Ning Yang1, Jie Zhou1, Fengying Yuan1

  • 1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 19, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces an adaptive threshold for DNA-based cryptography, improving message authenticity. This method enhances reliability in biological environments by using dynamic networks for accurate data decoding.

Keywords:
DNA decryptionDNA nanotechnologyDNA self‐assemblyDNA sensingnanomachine

More Related Videos

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K

Related Experiment Videos

Last Updated: Jun 16, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly
09:34

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

7.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cryptography

Background:

  • Nucleic acid-based cryptography offers innovative information processing but suffers from poor reproducibility and bioenvironmental interference.
  • Existing fixed-threshold decryption methods lead to binary translation errors, compromising message authenticity.

Purpose of the Study:

  • To develop an adaptive threshold mechanism for DNA constitutional dynamic networks (CDNs) to enhance the accuracy and authenticity of nucleic acid-based cryptography.
  • To overcome the limitations of fixed thresholds in variable bioenvironments.

Main Methods:

  • A programmed DNA constitutional dynamic network (CDN) was engineered to generate an adaptive threshold based on output pattern differences.
  • Self-calibrating patterns and constant constituent concentrations within the CDN system were implemented to minimize data errors.
  • Computational simulations were used to predict CDN system behavior.
  • The CDN system controlled orthogonal and cascaded nanoparticle-based molecular amplifiers for message expansion and DNA sensing.

Main Results:

  • The adaptive threshold successfully avoided contrary binary translations caused by slight output variations under fixed thresholds.
  • The self-calibrating patterns and constant CDN constituent concentrations significantly reduced data errors.
  • The system demonstrated effective coding and decoding of various messages, expanding transmitting message volume.
  • Accurate and specific DNA sensing was achieved.

Conclusions:

  • The developed adaptive threshold CDN system significantly improves the reliability and authenticity of nucleic acid-based cryptography.
  • This approach offers a robust solution for information processing in complex bioenvironments.
  • The CDN system shows promise for advanced molecular communication and sensing applications.