Electrochemical Immunosensor for C-Reactive Protein Detection Using an Optimized Bioinspired Ni-Doped Copper Ferrite...

Area of Science:

• Nanomaterials Science
• Electrochemistry
• Biomedical Engineering

Background:

• Cardiovascular diseases (CVDs) are the leading global cause of mortality.
• C-reactive protein (CRP) is a critical biomarker for early detection of cardiac events.
• Existing CRP detection methods require improvement in sensitivity, selectivity, and portability.

Purpose of the Study:

• To develop a highly sensitive, selective, and portable electrochemical biosensor for C-reactive protein (CRP) detection.
• To synthesize and characterize novel nickel-doped copper ferrite nanoparticles (Ni_(0.10)CuFe₂O₄ NPs).
• To evaluate the performance of the developed biosensor for CRP quantification.

Main Methods:

• Synthesis of nickel-doped copper ferrite nanoparticles using a hydrothermal method with Magnolia grandiflora leaf extract.
• Characterization of nanoparticles using XRD, FT-IR, UV, SEM, EDX, and HR-TEM/TEM.
• Fabrication of the electrochemical biosensor by depositing nanoparticles onto an indium tin oxide (ITO) surface via electrophoretic deposition (EPD) and electrochemical impedance spectroscopy (EIS) for sensing.

Main Results:

• The synthesized Ni_(0.10)CuFe₂O₄ NPs were successfully characterized.
• The electrochemical biosensor exhibited linear detection of CRP in the range of 0.5 to 500 ng/mL.
• High sensitivity was achieved with limits of detection (LOD) as low as 0.038 ng/mL and 0.033 ng/mL.

Conclusions:

• Nickel-doped copper ferrite nanoparticles form a promising platform for developing advanced electrochemical biosensors.
• The developed biosensor demonstrates significant potential for sensitive and early detection of CRP, aiding in cardiovascular disease monitoring.
• This technology offers a pathway towards more accessible and portable diagnostic tools for critical health biomarkers.