TFEBexplorer: An integrated tool to study genes regulated by the stress-responsive Transcription Factor EB

Area of Science:

• Molecular Biology
• Genetics
• Bioinformatics

Background:

• The transcription factor EB (TFEB) is a master regulator of lysosomal biogenesis and autophagy.
• Understanding TFEB's regulatory network is crucial for studying cellular homeostasis and disease.

Purpose of the Study:

• To develop TFEBexplorer, a comprehensive web tool for studying genes modulated by TFEB.
• To integrate diverse datasets including genetic perturbations and TFEB binding sites across species.

Main Methods:

• Integration of multiple datasets related to TFEB activity.
• Analysis of gene expression changes following TFEB manipulation (over-expression, knock-down, knock-out).
• Identification of experimentally validated and bioinformatically predicted TFEB binding sites.

Main Results:

• TFEBexplorer provides an intuitive interface to query genes of interest.
• Results are displayed in easy-to-interpret panels showing expression changes and binding site information.
• The database includes data across various cell lines and species.

Conclusions:

• TFEBexplorer serves as a valuable resource for researchers investigating TFEB-mediated gene regulation.
• The tool facilitates a deeper understanding of TFEB's role in cellular processes and disease.
• It offers a consolidated view of TFEB's impact on gene expression and binding.