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Related Experiment Video

Updated: May 23, 2025

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

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Published on: June 3, 2014

Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70.

Ke Lin^1,2,3, Wenjie Wei^2,4, Songzan Chen^1,2,3

¹Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Cell Death & Disease

|May 21, 2025

Summary

Asb10 exacerbates cardiac hypertrophy by stabilizing HSP70, a key factor in heart failure development. Inhibiting Asb10 may offer a therapeutic strategy for pressure overload-induced heart conditions.

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Area of Science:

Cardiovascular Biology
Molecular Cardiology
Heart Failure Pathogenesis

Background:

Cardiac hypertrophy is a major risk factor for heart failure.
Hypertension-induced pressure overload is a common trigger for left ventricular hypertrophy.
The ubiquitin-proteasome system's role in cardiac hypertrophy is increasingly recognized.

Purpose of the Study:

To investigate the role of Asb10, a heart tissue-enriched E3 ligase, in cardiac hypertrophy and heart failure.
To elucidate the molecular mechanisms by which Asb10 influences cardiac hypertrophy.

Main Methods:

Bioinformatic screening of GEO datasets and experimental validation.
In vitro studies using NRVMs with adenoviral Asb10 overexpression.
Immunoprecipitation-mass spectrometry and co-immunoprecipitation assays.
In vivo studies in mice subjected to Transverse Aortic Constriction (TAC) surgery.

Main Results:

Asb10 was identified as downregulated in cardiac hypertrophy.
Asb10 overexpression exacerbated hypertrophic growth and worsened cardiac function post-TAC.
Asb10 stabilizes HSP70 by blocking STUB1-mediated ubiquitination and degradation.
Elevated HSP70, cardiac inflammation, and pHDAC2^S394 activation were linked to Asb10's effects.

Conclusions:

Asb10 exacerbates cardiac hypertrophy by stabilizing HSP70 through competitive inhibition of STUB1.
Asb10 plays a significant role in hemodynamic stress-induced cardiac hypertrophy and heart failure.
Targeting Asb10 or HSP70 may represent a therapeutic approach for heart failure.