Urokinase-type plasminogen activator in ascites obtained from the patient with mammary cancer

Plasminogen activator (ascites PA) was purified from the ascites of the patient with breast cancer using lysine-Sepharose, salting out, DEAE Sephadex A-50 and anti-urokinase (UK) Sepharose chromatography. The ascites PA was adsorbed on anti-UK Sepharose and its activity was neutralized by anti-UK IgG fraction. The molecular weights of the ascites PA proteins were 70,000, 32,000 and 25,000 daltons. The ascites PA activated plasminogen and showed amidolytic activity for S-2444. Zymograms of ascites PA showed that 70,000 and 32,000 daltons proteins had a PA activity. The ascites PA thus contained two types of PAs, one was similar to LMW-UK and the other was an UK type tissue PA.