Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Differential self-assembly of sequence-isomeric phosphoestamers.

Chemical communications (Cambridge, England)·2026

Same author

Dissecting the Effects of Cage Structure in the Catalytic Activation of Imide Chlorenium-Ion Donors.

Journal of the American Chemical Society·2025

Same author

Control of a Gel-Forming Chemical Reaction Network Using Light-Triggered Proton Pumps.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

<i>Metallicious</i>: Automated Force-Field Parameterization of Covalently Bound Metals for Supramolecular Structures.

Journal of chemical theory and computation·2024

Same author

Tips and Tricks in the Modeling of Supramolecular Peptide Assemblies.

ACS omega·2024

Same author

CageCavityCalc (<i>C</i>3): A Computational Tool for Calculating and Visualizing Cavities in Molecular Cages.

Journal of chemical information and modeling·2024

Same journal

Ambient stability and surface adhesion of 2D polyaramid nanofilms.

Faraday discussions·2026

Same journal

Spiers Memorial Lecture: Spin-mediated promotion of magnetic metal catalysts.

Faraday discussions·2026

Same journal

Helium spin-echo as a surface-sensitive probe of vibrational energy dissipation.

Faraday discussions·2026

Same journal

Near-infrared vibrational second harmonic generation: a new nonlinear interfacial vibrational spectroscopy.

Faraday discussions·2026

Same journal

CO on a Rh/Fe<sub>3</sub>O<sub>4</sub> single-atom catalyst: high-resolution infrared spectroscopy and near-ambient-pressure scanning tunnelling microscopy.

Faraday discussions·2026

Same journal

Evolution of size-selected Pt cluster catalysts on prototypical oxide supports.

Faraday discussions·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 20, 2025

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Published on: January 16, 2014

Fiber formation seen through the high-resolution computational microscope.

Tomasz K Piskorz¹, Vasudevan Lakshminarayanan¹, Alex H de Vries²

¹Department of Chemical Engineering, Delft University of Technology, van der Maasweg 9, Delft, 2629 HZ, The Netherlands. tomasz.k.piskorz@gmail.com.

Faraday Discussions

|May 23, 2025

Summary

This summary is machine-generated.

This study reveals how supramolecular fibers form, from initial monomer collapse to bundling. Understanding these self-assembly dynamics is key for designing new materials.

More Related Videos

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Related Experiment Videos

Last Updated: Sep 20, 2025

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Published on: January 16, 2014

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

Published on: January 21, 2019

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Area of Science:

Supramolecular chemistry
Materials science
Biophysics

Background:

Supramolecular fibers are crucial in biological systems and advanced materials.
Understanding fiber formation requires detailed energy landscape insights.
The link between molecular building blocks and final structure is complex.

Purpose of the Study:

To investigate the multi-stage formation of 1,3,5-cyclohexanetricarboxamide fibers.
To elucidate the dynamics of nucleation, elongation, secondary nucleation, and bundling.
To map the energy landscape of supramolecular fiber assembly.

Main Methods:

Utilized Markov state modeling of molecular dynamics simulations.
Employed the polarizable CHARMM Drude force field for accurate modeling.
Simulations covered timescales up to microseconds.

Main Results:

Observed rapid monomer collapse during nucleation, forming disordered assemblies that mature into nuclei.
Identified elongation and secondary nucleation as competing processes influenced by monomer density.
Bundling involves initial fiber association followed by stabilization through surface reorganization.

Conclusions:

Provided comprehensive insights into supramolecular fiber formation stages.
Demonstrated the critical role of monomer density in competing growth mechanisms.
Revealed the mechanism of bundle stabilization via surface interactions and aromatic group interactions.