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Porous biocompatible composite scaffold (CS/MFC/HAp) with N-Boc L-cysteine methyl ester for bone tissue engineering
Sivasankar Mv1, Sreenivasa Rao Parcha1
1Department of Biotechnology, Stem Cell Research Laboratory, National Institute of Technology, Warangal, Telangana, India.
Journal of Biomaterials Science. Polymer Edition
|May 24, 2025
Summary
This study developed novel composite scaffolds using micro-fibrillated cellulose (MFC), chitosan (CS), and hydroxyapatite (HAp), enhanced with N-Boc-L-cysteine methyl ester (NBLCME). These scaffolds show promise for bone regeneration applications due to their structure and biological activity.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Materials Chemistry
Background:
- Developing advanced biomaterials is crucial for bone regeneration.
- Composite scaffolds offer tunable properties for orthopedic applications.
- Integrating bioactive molecules can enhance scaffold performance.
Purpose of the Study:
- To fabricate and characterize novel composite scaffolds (CS/MFC/HAp) incorporating N-Boc-L-cysteine methyl ester (NBLCME).
- To evaluate the drug delivery kinetics and biological performance of the NBLCME-loaded scaffolds for bone-related applications.
Main Methods:
- Fabrication of CS/MFC/HAp composite scaffolds using freeze-drying.
- Incorporation of NBLCME at varying concentrations (20-100µg/ml).
- Characterization using SEM, FTIR, porosity, and pore size analysis.
- In vitro biological evaluation on MG63 cells (cytotoxicity, ALP, ARS, cell adhesion).
Main Results:
- Scaffolds exhibited interconnected porous structures (80-90% porosity, 100-450µm pores).
- FTIR confirmed successful incorporation and interaction of NBLCME with the scaffold matrix.
- Sustained drug release consistent with Fickian diffusion was observed.
- Enhanced cell viability, ALP, and ARS activities indicated improved osteogenic potential without cytotoxicity.
Conclusions:
- The fabricated CS/MFC/HAp composite scaffolds loaded with NBLCME possess favorable structural and biological properties.
- These scaffolds demonstrate significant potential for bone regeneration and related orthopedic applications.
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