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Related Experiment Videos

A fast cell sampler for flow cytometry.

F L Battye, W Darling, J Beall

    Cytometry
    |September 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    A new, simple device enhances flow cytometry by efficiently delivering small sample volumes. This cost-effective sampler increases analytical throughput to over 120 samples per hour.

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    Area of Science:

    • Biotechnology
    • Analytical Chemistry
    • Cell Biology

    Background:

    • Flow cytometry is a crucial technique for cell analysis.
    • High sample throughput is essential for efficient laboratory workflows.
    • Existing sample delivery methods can be complex or limited in flexibility.

    Purpose of the Study:

    • To develop a simple, economical, and flexible device for sample delivery into a flow cytometer.
    • To improve the analytical throughput of existing flow cytometer systems.

    Main Methods:

    • A novel device featuring a lever-operated disc valve was designed.
    • The device allows loading of small sample volumes (≥20 microliters) into a tubing loop.
    • Samples are injected into the cytometer nozzle using saline pressure.

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    Main Results:

    • The device demonstrated simplicity with only one moving part.
    • It accommodates various cell sample containers and concentrations.
    • The maximum analytical throughput of a FACS II cell sorter was increased to over 120 samples per hour.

    Conclusions:

    • The developed device offers an efficient and cost-effective solution for sample delivery in flow cytometry.
    • This innovation significantly enhances the analytical throughput of cell sorters.
    • The device's flexibility makes it suitable for a wide range of applications in cell analysis.