Related Experiment Video
Updated: Feb 27, 2026
07:59
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
10.5K
Two new abietane diterpenoids from Pinus massoniana.
Xue Ma1,2,3, Zhi-Long He1,2,4, Bao Zhang5
1School of Basic Medical Sciences, Guizhou Medical University, Guian New Area 561113, China.
Journal of Asian Natural Products Research
|May 28, 2025
Summary
Two novel abietane diterpenoids were isolated from Pinus massoniana. Massonianene B demonstrated significant inhibition of nitric oxide production in immune cells.
Area of Science:
- Natural Products Chemistry
- Organic Chemistry
- Pharmacology
Background:
- Pinus massoniana is a traditional medicinal plant with a rich source of bioactive compounds.
- Abietane diterpenoids are a class of natural products known for diverse biological activities.
Purpose of the Study:
- To isolate and characterize new abietane diterpenoids from Pinus massoniana.
- To evaluate the anti-inflammatory potential of the isolated compounds.
Main Methods:
- Phytochemical investigation of Pinus massoniana extract.
- Structure elucidation using High-Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), Infrared (IR) spectroscopy, Ultraviolet (UV) spectroscopy, Nuclear Magnetic Resonance (NMR) spectroscopy, Electronic Circular Dichroism (ECD), and X-ray diffraction analysis.
- In vitro anti-inflammatory assay using lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells.
Main Results:
- Two new abietane diterpenoids, named massonianene A (1) and massonianene B (2), were successfully isolated and identified.
- The chemical structures of massonianene A and B were rigorously determined through comprehensive spectroscopic and crystallographic analyses.
- Massonianene B (2) exhibited significant inhibitory activity against LPS-induced NO production in RAW 264.7 cells, with a potent IC50 value of 7.00 ± 1.73 μM.
Conclusions:
- The study successfully identified two novel abietane diterpenoids from Pinus massoniana.
- Massonianene B possesses notable anti-inflammatory properties, suggesting its potential as a therapeutic agent for inflammatory conditions.
More Related Videos
Related Concept Videos
01:16Adrenergic Agonists: Chemistry and Structure-Activity Relationship
4.0K
Adrenergic agonists' structure-activity relationship (SAR) determines their selectivity and efficacy. These agonists comprise a phenylethylamine moiety with an aromatic ring and an ethylamine side chain.
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
4.0K
01:20¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K
01:28Adrenergic Agonists: Mixed-Action Agents
1.6K
Mixed-action adrenergic agonists, like ephedrine and pseudoephedrine, directly and indirectly affect adrenergic receptors. These agents stimulate adrenoceptors and indirectly release stored neurotransmitters, amplifying the adrenergic response.
Ephedrine and pseudoephedrine lack a catecholamine group, making them less susceptible to degradation by metabolic enzymes. They have increased oral bioavailability and lipophilicity, resulting in a longer duration of action. Their response is reduced by...
Ephedrine and pseudoephedrine lack a catecholamine group, making them less susceptible to degradation by metabolic enzymes. They have increased oral bioavailability and lipophilicity, resulting in a longer duration of action. Their response is reduced by...
1.6K