Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

3.9K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.9K
ER Retrieval Pathway01:45

ER Retrieval Pathway

3.9K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
The Early Endosome: Endocytosis of Transferrin01:28

The Early Endosome: Endocytosis of Transferrin

3.5K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.5K
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

7.4K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.4K
Rab Cascades01:25

Rab Cascades

2.7K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifaceted Roles of Retromer in EGFR Trafficking and Signaling Activation.

Cells·2022
Same author

De novo macrocyclic peptides for inhibiting, stabilizing, and probing the function of the retromer endosomal trafficking complex.

Science advances·2021
Same author

How size, edge shape, functional groups and embeddedness influence the electronic structure and partial optical properties of graphene nanoribbons.

Physical chemistry chemical physics : PCCP·2021
Same author

NIR-II-driven and glutathione depletion-enhanced hypoxia-irrelevant free radical nanogenerator for combined cancer therapy.

Journal of nanobiotechnology·2021
Same author

PSPC1 regulates CHK1 phosphorylation through phase separation and participates in mouse oocyte maturation.

Acta biochimica et biophysica Sinica·2021
Same author

A Robust Hypoxia Risk Score Predicts the Clinical Outcomes and Tumor Microenvironment Immune Characters in Bladder Cancer.

Frontiers in immunology·2021

Related Experiment Video

Updated: Sep 20, 2025

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

9.2K

Disruption of Retriever Function Impacts Retrograde Trafficking From Endosomes.

Zebin Li1, Zhe Yang1, Rohan D Teasdale1

  • 1School of Biomedical Sciences, Faculty of Medicine, The University of Queensland, Brisbane, Australia.

Cell Biology International
|May 29, 2025
PubMed
Summary

The retriever complex is essential for efficient endosomal retrograde trafficking, a process distinct from retromer

Keywords:
endosomesmembrane transportprotein traffickingretrieverretrograde trafficking

More Related Videos

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.7K
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

9.7K

Related Experiment Videos

Last Updated: Sep 20, 2025

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
11:05

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Published on: February 21, 2019

9.2K
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
08:51

The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking

Published on: February 12, 2022

3.7K
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

9.7K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • Endosomal sorting and packaging into endosomal-transport carriers (ETCs) direct protein delivery.
  • Retromer complex mediates ETC formation for retrograde trafficking and recycling.
  • The retriever complex, similar to retromer, recycles cargo but its retrograde role is unknown.

Purpose of the Study:

  • Investigate the role of the retriever complex in endosomal retrograde trafficking.
  • Compare the functions of retromer and retriever in protein sorting and ETC formation.
  • Elucidate the distinct mechanisms of retrograde ETC initiation by these complexes.

Main Methods:

  • Generated CRISPR-mediated retromer and retriever knockout (KO) A549 cell models.
  • Confirmed retriever's role in Integrin β1 recycling.
  • Utilized ETC redirection assays and cargo redistribution analysis.

Main Results:

  • Retriever KO cells showed decreased retrograde trafficking of cation-independent mannose 6-phosphate receptor and TGN46.
  • Golgin97 and Golgin245 tethered ETCs dependent on either retromer or retriever.
  • Retriever recruitment to endosomes is dependent on retromer, suggesting distinct mechanisms.

Conclusions:

  • Retriever is required for efficient endosomal retrograde trafficking.
  • The mechanisms initiating retrograde ETC formation by retromer and retriever appear distinct.
  • Retromer is required for WASH recruitment, but retriever is not.