Ratiometric sensing based on self-assembled dipeptide: Color-resolved visualization of quantified evaluation for...

Area of Science:

• Analytical Chemistry
• Materials Science
• Food Science

Background:

• Biogenic amines (BAs) are critical biomarkers for evaluating meat product freshness.
• Accurate and rapid detection of BAs is essential for food safety and quality control.
• Existing methods for BA detection can be time-consuming or lack sensitivity.

Purpose of the Study:

• To develop a novel fluorescent nanoprobe for sensitive and rapid detection of biogenic amines.
• To create a ratiometric sensing system for enhanced accuracy in meat spoilage assessment.
• To validate the probe's efficacy in monitoring meat spoilage under various conditions.

Main Methods:

• Synthesis of fluorescent nanoprobes using self-assembled dipeptides and methyl red.
• Development of a ratiometric fluorescence sensing strategy utilizing dual-emission peaks (400 and 610 nm).
• Testing the probe's performance for detecting specific biogenic amines (histamine, tyramine, putrescine, tryptamine, spermine) and its application in meat spoilage monitoring.

Main Results:

• The ratiometric fluorescent probe demonstrated dual-emission peaks, minimizing environmental and instrumental interference.
• Low detection limits for key biogenic amines were achieved within a 12-minute detection time.
• The probe effectively monitored meat spoilage at different temperatures, showing practical applicability.

Conclusions:

• The developed self-assembled dipeptide-based fluorescent nanoprobe provides a sensitive, rapid, and accurate method for biogenic amine detection.
• This ratiometric sensing approach offers a robust tool for assessing meat freshness and detecting spoilage.
• The test strips show significant potential for practical, on-site food quality monitoring.