Ex Vivo Analysis of Single Cancer Cell Homing to Murine Bone via Two-Photon Microscopy
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Updated: Oct 8, 2026

Tracking Mouse Bone Marrow Monocytes In Vivo
Published on: February 27, 2015
Gloria Allocca1, Ning Wang2,3
1Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Milan, Italy. gloria.allocca@ieo.it.
Abstract:
Two-photon microscopy has gained widespread recognition as a potent tool for providing both qualitative and quantitative information in the field of bone research. This chapter outlines a detailed protocol for using two-photon microscopy to track the colonization of cancer cells to bone, using frozen bone samples derived from xenograft mouse models.
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