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Related Concept Videos

Tight Junctions01:29

Tight Junctions

5.2K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
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Anchoring Junctions01:03

Anchoring Junctions

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Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
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Adherens Junctions01:24

Adherens Junctions

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Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
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Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

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The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

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In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
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Related Experiment Video

Updated: Jun 16, 2025

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor
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Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

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LAP-Hsp60 complex modulates epithelial tight junction barrier.

Arun Bhunia1,2, Manalee Manalee Samaddar2,3, Chen Sun1,2

  • 1Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.

Research Square
|June 5, 2025
PubMed
Summary
This summary is machine-generated.

Listeria adhesion protein (LAP) and Hsp60 form a complex that enhances intestinal drug absorption. This discovery offers a new strategy for delivering biologics orally without adverse effects.

Keywords:
Hsp60LAPcryo-EMmousepeptide drug deliverystructure-functiontight junction modulator

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Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
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Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
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Area of Science:

  • Biochemistry
  • Structural Biology
  • Pharmacology

Background:

  • Listeria adhesion protein (LAP) modulates tight junctions (TJs) by interacting with Hsp60, aiding Listeria monocytogenes translocation.
  • Existing TJ modulators (TJMs) have shown variable and limiting effects.

Purpose of the Study:

  • To structurally characterize the LAP-Hsp60 complex.
  • To evaluate purified LAP as a TJM for enhancing oral drug delivery.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) and computational analysis were used to determine the structure of LAP alone and in complex with Hsp60.
  • A mouse model was employed to assess LAP-mediated drug absorption.

Main Results:

  • The LAP structure revealed multimeric interlocking dimers and tetramers, with the N-domain interacting with Hsp60 and the C-domain binding InlB.
  • LAP facilitated the absorption of vancomycin and desmopressin across the intestinal barrier without causing inflammation or TJ damage.

Conclusions:

  • The LAP-Hsp60 complex is structurally defined and functions as an effective TJM.
  • LAP and its mimetics hold promise for improving peroral biologics delivery.