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Calcium Carbonate Formation in the Presence of Biopolymeric Additives

Calcium Carbonate Formation in the Presence of Biopolymeric Additives

Published on: May 14, 2019

Glycated walnut peptide-calcium chelates: Structural properties, calcium absorption enhancement mechanism, and

Zilin Wang¹, Haifen Jiang¹, Jiahe Dai¹

¹College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.

|June 7, 2025

Abstract:

This study examines the structural characteristics of a novel glycated walnut peptide‑calcium chelate (COS-AERGVLYR-Ca), explores its mechanisms of calcium absorption and transport, and investigates its impact on osteoblast MC3T3-E1 cells. Computer simulation results indicate that stable binding is formed between calcium ions and the carbonyl oxygen of leucine, carboxyl oxygen of glutamic acid, and carbonyl oxygen of valine in AERGVLYR. Further structural characterization confirms that Ca²⁺ bind to the peptides through carboxyl oxygen and amino nitrogen, and glycation modification enhances the structural stability of AERGVLYR, exhibiting robust inhibition of calcium phosphate crystallization and gastrointestinal stability. Furthermore, COS-AERGVLYR effectively resisted the inhibition of anti-nutritional factors on Ca²⁺ absorption and facilitated calcium ion transport via TRPV6 and CAV1.3 channels, significantly promoting the proliferation and mineralization of osteoblast MC3T3-E1 cells. This research provides a theoretical basis for the development of glycated peptide-based calcium supplements, offering a promising new avenue for enhancing calcium bioavailability and promoting calcium absorption.

Keywords:

Caco-2 cell transport Glycated peptide‑calcium chelates Osteoblast proliferation Stability Structural characterization

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