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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Sep 19, 2025

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Published on: October 29, 2020

DNA Hanger: Surface-Minimized Single-Molecule Immunoassay Platform.

Jincheol Seol¹, Byungju Kim², Eui-Sang Yu³

¹Division of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science & Technology (POSTECH), Pohang, 37673, South Korea.

Small (Weinheim an Der Bergstrasse, Germany)

|June 8, 2025

Summary

This summary is machine-generated.

A new DNA Hanger assay offers sensitive single-molecule protein detection with minimal background noise. This innovative platform simplifies immunoassays and shows potential for clinical diagnostics.

Keywords:

DNA skybridge single‐molecule FLISA single‐molecule immunoassay

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The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Area of Science:

Biochemistry
Molecular Biology
Assay Development

Background:

Conventional surface-based immunoassays suffer from limitations including high background noise and complex workflows.
There is a need for highly sensitive and specific protein detection methods with reduced nonspecific binding.

Purpose of the Study:

To develop a novel single-molecule immunoassay platform, termed DNA Hanger, to overcome limitations of existing assays.
To demonstrate the specificity and sensitivity of the DNA Hanger platform for protein quantification.

Main Methods:

The DNA Hanger platform suspends biotinylated DNA across microfabricated quartz barriers to capture target proteins.
Single-molecule detection was achieved using fluorescence-based quantification.
The assay was validated using mNeonGreen-tagged human poly(A)-binding protein C1 and human tumor necrosis factor α (TNF-α).

Main Results:

DNA Hanger achieved significantly reduced nonspecific binding rates (one protein per 236 µm DNA).
The assay demonstrated high sensitivity, with a detection limit of 0.90 pM for TNF-α in buffer (38-fold improvement over conventional FLISA).
Detection in 70% fetal bovine serum showed an 8-fold improvement, with a limit of 20.6 pM, and enabled endogenous TNF-α detection in human serum.

Conclusions:

The DNA Hanger platform provides a novel approach for highly specific and sensitive single-molecule protein detection.
The simplified workflow and rapid assay completion (within 1 hour) highlight its practical advantages.
DNA Hanger shows broad applicability for biomolecular interaction studies and holds significant potential for clinical diagnostics.