Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Interfacial Processes in Single Hydrogen Microbubbles Probed by Operando Opto-Electrochemistry.

Analytical chemistry·2026
Same author

Nanopore-Enabled Dark-Field Digital Sensing of Nanoparticles.

Analytical chemistry·2023
Same author

Calcite-Assisted Localization and Kinetics (CLocK) Microscopy.

The journal of physical chemistry letters·2022
Same author

Electrochemical Zero-Mode Waveguide Potential-Dependent Fluorescence of Glutathione Reductase at Single-Molecule Occupancy.

Analytical chemistry·2022
Same author

Electrochemical and spectroelectrochemical characterization of bacteria and bacterial systems.

The Analyst·2021
Same author

Actively Controllable Solid-Phase Microextraction in a Hierarchically Organized Block Copolymer-Nanopore Electrode Array Sensor for Charge-Selective Detection of Bacterial Metabolites.

Analytical chemistry·2021
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2025

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
15:39

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

8.2K

Electrochemical Calcite-Assisted Localization and Kinetics (E-CLocK) Microscopy.

Ivani Jayalath1, Shubhendra Shukla1, Govinda Anantha Padmanabha1

  • 1Department of Chemistry and Biochemistry, University of Mississippi, University, Mississippi 38655, United States.

Analytical Chemistry
|June 11, 2025
PubMed
Summary
This summary is machine-generated.

A new microscopy technique, E-CLocK, tracks nanoparticle shape changes during electrochemical reactions in real-time. This method reveals how surfactants like CTAB influence nanoparticle growth, promoting anisotropic shapes and advancing structure-activity studies.

More Related Videos

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
09:30

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

10.7K
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

8.9K

Related Experiment Videos

Last Updated: Jun 13, 2025

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
15:39

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm

Published on: February 28, 2017

8.2K
Imaging Local Ca2+ Signals in Cultured Mammalian Cells
09:30

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

10.7K
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

8.9K

Area of Science:

  • Electrochemistry
  • Materials Science
  • Microscopy

Background:

  • Nanoparticle (NP) morphology critically impacts electrochemical reaction efficiency and selectivity.
  • Conventional electrochemical methods lack dynamic NP morphological change insights.
  • Advanced microscopy (AFM, TEM) is costly and can interfere with electrochemical measurements.

Purpose of the Study:

  • Introduce a novel, non-invasive super-resolution microscopy technique for real-time NP morphological tracking during electrochemical processes.
  • Enable quantitative and qualitative analysis of single-nanoparticle morphological dynamics.
  • Advance single-particle structure-activity relationship studies in electrochemistry.

Main Methods:

  • Developed dark-field-based electrochemical calcite-assisted localization and kinetics (E-CLocK) microscopy.
  • Integrated a rotating calcite crystal into a dark-field microscope for super-resolution imaging.
  • Analyzed point spread functions to determine NP anisotropy and orientation during electrodeposition.

Main Results:

  • Quantitatively assessed morphological anisotropy of individual gold NPs during electrodeposition.
  • Observed steady isotropic growth with gold precursor alone.
  • Demonstrated that CTAB surfactant induced oscillatory behavior and promoted anisotropic NP growth.

Conclusions:

  • E-CLocK microscopy offers high-throughput, reliable tracking of morphological changes during electrochemical reactions.
  • The technique significantly advances the study of single-particle structure-activity relationships.
  • Revealed the significant influence of surfactants on NP morphology during electrochemical synthesis.