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Updated: Jun 13, 2025
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Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
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Comparative Evaluation of Three Calcium Silicate-Based Materials for Direct Pulp Capping: An In Vivo Study in Mice
Fatma Fenesha1, Aonjittra Phanrungsuwan1, Dane Kanniard1
1Division of Biosciences, College of Dentistry, The Ohio State University, Columbus, OH, USA.
Biorxiv : the Preprint Server for Biology
|June 12, 2025
Summary
Ready-to-use bioceramic materials show promise for direct pulp capping (DPC) in mice. These agents effectively seal cavities and promote dentin bridge formation, crucial for preserving pulp vitality.
Area of Science:
- Biomaterials Science
- Dental Research
- Regenerative Dentistry
Background:
- Vital pulp treatments (VPT) aim to preserve pulp vitality, but direct pulp capping (DPC) success is debated due to sealing challenges.
- Limitations of traditional materials like MTA have driven the development of new calcium silicate-based agents.
- Comprehensive assessment of reparative dentin formation and sealing ability is needed for long-term clinical success.
Purpose of the Study:
- To optimize a DPC technique in mice.
- To compare the tissue response and sealing ability of three calcium silicate agents (Bioceramic putty, Biodentine, Theracal-LC) against a control (Cavit-G).
Main Methods:
- Direct pulp capping performed on maxillary first molars of C57BL/6 female mice.
- Reparative dentin assessed via micro-CT and histology at 21 days.
- Sealing ability evaluated using micro-CT to quantify material voids.
Main Results:
- All tested calcium silicate materials demonstrated good biocompatibility and induced dentin bridge formation.
- Bioceramic putty and Theracal-LC showed superior sealing ability compared to Biodentine, with fewer voids detected by micro-CT.
- Cavit-G served as a control for sealing but caused desiccation.
Conclusions:
- Ready-to-use, premixed calcium silicate agents offer superior sealing in a mouse DPC model.
- These materials effectively promote dentin bridge formation, supporting pulp protection and vitality.
- The findings suggest potential for these advanced materials in clinical direct pulp capping procedures.
