Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
RNA Structure01:19

RNA Structure

4.7K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
4.7K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
RNA Stability01:53

RNA Stability

33.4K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

875
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
875
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Replication-stress-induced chromatin loops protect fork stability.

Nature·2026
Same author

Valence-Arousal Asymmetry: Rethinking Facial Emotion Recognition in Preschool Autism Through Eye-Tracking.

Autism research : official journal of the International Society for Autism Research·2026
Same author

A Novel Framework for Quantitative Evaluation of Resilience Performance of Sea Lanes of Communication.

Risk analysis : an official publication of the Society for Risk Analysis·2026
Same author

Amelioration of tic disorder by Jujuboside A via gut microbiota remodeling and intestinal 5-HT signaling.

Frontiers in neuroscience·2026
Same author

Comparative Analysis of the In Vitro and In Vivo Antioxidant and Anti-Inflammatory Capacities of Lycopene Extracts from Different Sources.

Foods (Basel, Switzerland)·2026
Same author

An Overlooked Formulation Variable: Solvent Reshapes DNA Delivery.

ACS macro letters·2026
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Jun 14, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

398

RNA transcripts regulate G-quadruplex landscapes through G-loop formation.

Koichi Sato1,2, Jing Lyu3,4, Jeroen van den Berg1

  • 1Oncode Institute, Hubrecht Institute-KNAW & University Medical Center Utrecht, Utrecht, Netherlands.

Science (New York, N.Y.)
|June 12, 2025
PubMed
Summary
This summary is machine-generated.

RNA transcripts control DNA G-quadruplex (G4) structures via a G-loop mechanism. This process involves coordinated assembly and disassembly, crucial for maintaining genome stability and cellular survival.

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

7.9K
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.6K

Related Experiment Videos

Last Updated: Jun 14, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

398
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

7.9K
A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Genomics

Background:

  • G-quadruplexes (G4s) are DNA structures regulating transcription but can compromise genome stability.
  • The precise mechanisms controlling G4 dynamics are not fully understood.

Purpose of the Study:

  • To elucidate how RNA transcripts regulate G4 structures.
  • To investigate the G-loop mechanism controlling G4 dynamics.

Main Methods:

  • Investigated G-loop assembly involving ATM/ATR kinases, BRCA2, and RAD51.
  • Studied G-loop disassembly mediated by DHX36-FANCJ.
  • Analyzed the impact of inhibiting G-loop disassembly on cellular processes.

Main Results:

  • RNA transcripts orchestrate G4 landscapes via G-loop assembly and disassembly.
  • G-loop assembly requires ATM/ATR kinases and BRCA2/RAD51 for RNA-DNA invasion.
  • G-loop disassembly involves DHX36-FANCJ unwinding, nucleolytic incision, and DNA synthesis.

Conclusions:

  • A novel G-loop assembly-disassembly mechanism controls G4 structures.
  • Disruption of G-loop disassembly leads to G4/R-loop accumulation, transcriptome dysregulation, and genome instability.
  • This mechanism is vital for cellular homeostasis and survival.